•Supplemental Videos•

Please visit the News section of our website for further reading on the Lieberman Lab
and commentaries on our publications.

Grant R, Hamer D, Hope Thomas, Johnston R, Lange J, Lederman M, Lieberman J, Miller C, Moore J, Mosier D, Richman D, Schooley R, Springer M, Veazey R, Wainberg M. Whither or Wither Microbicides? Science 2008; 321:532-534. [PDF]

Chowdhury D, Xu X, Zhong X, Ahmed F, Zhong J, Liao J, Dykxhoorn DM, Weinstock D, Pfeifer G, and Lieberman J. A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication. Mol Cell 2008 Jul 11;31(1):33-46. [PDF]

Martinvalet D, Dykxhoorn DM, Ferrini R, Lieberman J. Granzyme A Cleaves a Mitochondrial Complex I Protein to Initiate Caspase-Independent Cell Death. Cell 2008 133:681-692. [PDF]

Chowdhury D, Lieberman J. Death by a Thousand Cuts: Granzyme Pathways of Programmed Cell Death. Annu Rev Immunol 2008; 26:389-420. [PDF]

Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ: Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen. Science 2007; 319:921-926. [PDF]

F Yu, H Yao, P Zhu, X Zhang, Q Pan, C Gong, Y Huang, X Hu, F Su, J Lieberman, E Song, "let-7 Regulates Self Renewal and Tumorigenicity of Breast Cancer Cells," Cell 131:1109-1123, 2007. [PDF]

MA Lee-Kirsch, M Gong, D Chowdhury, L Senenko, K Engel, Y-A Lee, U de Silva, SL Bailey, T Witte, TJ Vyse, J Kere, C Pfeiffer, S Harvey, A Wong, S Koskenmies, O Hummel, K Rohde, RE Schmidt, AF Dominiczak, M Gahr, T Hollos, FW Perrino, J Lieberman, N Hubner, "Mutations in the 3'-5' DNA exonuclease TREX1 are associated with systemic lupus erythematosis," Nature Genetics 39(9): 1065-1067, 2007. [PDF]

MA Lee-Kirsch, D Chowdhury, S Harvey, M Gong, L Senenko, K Engel, C Pfeiffer, T Hollis, FW Perrino, J Lieberman, N Hubner, "A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupus," J Mol Med 85:531-537, 2007. [PDF]

AM Glodek, Y Le, DM Dykxhoorn, S-Y Park, G Mostoslavsky, R Mulligan, J Lieberman, M Honczarenko, L Silberstein, "Focal Adhesion Kinase (FAK) is required for CXCL12-induced chemotactic and pro-adhesive responses in hematopoietic precursor cells," Leukemia 21, 1723-1732, 2007. [PDF]

D Peer, P Zhu, C Carman, J Lieberman, M Shimaoka, "Selective gene silencing in activated leukocytes by targeting siRNAs to the integrin lymphocyte function-associated antigen 1," Proc Natl Acad Sci USA 104:4095-4100, 2007. [PDF]

A de Fougerolles, H-P Vornlocher, J Maraganore, J Lieberman, "Interfering with Disease: A Progress Report on siRNA-based Therapeutics," Nature Rev Drug Discovery 6: 443-453, 2007. [PDF]

ME Pipkin, J Lieberman, "Delivering the kiss of death: progress on understanding how perforin works," Curr Opin Immunol 19: 301-308, 2007. [PDF]

DM Dykxhoorn, J Lieberman, "Knocking down disease with siRNAs," Cell 126:231-235, 2006. [PDF]

DM Dykxhoorn, J Lieberman, "Silencing Viral Infection," PLOS Medicine 3:1000-1004, 2006. [PDF]

D Chowdhury, P Beresford, P Zhu, D Zhang, J-S Sung, B Demple, F Perrino, J Lieberman, "The Exonuclease TREX1 is in the SET Complex and Acts in Concert with NM23-H1 to Degrade DNA during Granzyme A-Mediated Cell Death," Mol Cell 23:133-142, 2006. [PDF]

S Ranjbar, AV Tsytsykova, S-K Lee, R Rajsbaum, JV Falvo, J Lieberman, P Shankar, A Goldfeld, "NFAT5 Regulates HIV-1 in Primary Monocytes via a Highly Conserved LTR Site," PLoS Pathogens 2:1176-1186, 2006. [PDF]

DM Dykxhoorn, L Schlehuber, I London, J Lieberman, "Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism," PNAS 103:5953-5958, 2006. [PDF]

P Zhu, D Zhang, D Chowdhury, D Martinvalet, D keefe, L Shi, J Lieberman, "Granzyme A, which causes single-stranded DNA damage, targets the double-strand break repair protein Ku 70," EMBO Rep 7:431-437, 2006. [PDF]

DM Dykxhoorn, D Palliser, J Lieberman, "The silent treatment: siRNAs as small molecule drugs," Gene Ther 2006. [PDF]

D Palliser, D Chowdhury, Q-Y Wang, SJ Lee, RT Bronson, DM Knipe, J Lieberman, "An siRNA-based microbicide protects mice from lethal herpes simplex virus 2 infection," Nature 439:89-94, 2006. [PDF]

MC Keogh, J-A Kim, M Downey, J Fillingham, D Chowdhury, JC Harrison, M Onishi, N Datta, S Galicia, A Emili, J Lieberman, X Shen, S Buratowski, JE Haber, D Durocher, JF Greenblatt, NJ Krogan, "A phosphatase complex that dephosphorylates yH2AX regulates DNA damage checkpoint recovery," Nature 439:497-501, 2006. [PDF]

Dykxhoorn DM, Lieberman J. Running Interference: Prospects and Obstacles to Using Small Interfering RNAs as Small Molecule Drugs. Annu Rev Biomed Eng 2006; 8:377-402. [PDF]

D Chowdhury, MC Keogh, H Ishii, CL Peterson, S Buratowski, J Lieberman, "y-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double strand break repair," Mol Cell 20:801-809, 2005. [PDF]

D Keefe, L Shi, S Feske, R Massol, F Navarro, T Kirchhausen, J Lieberman, "Perforin Triggers a plasma membrane-repair response that facilitates CTL induction of apoptosis," Immunity 23:249-262, 2005. [PDF]

S-K Lee, DM Dykxhoorn, P Kumar, S Ranjbar, E Song, L Maliszewski, V Francois-Bongarcon, AE Goldfeld, MN Swamy, J Lieberman, P Shankar, "Lentiviral delivery of short hairpin RNAs protects CD4 T cells from multiple clades and primary isolates of HIV-1," Blood 106:818-826, 2005. [PDF]

AL Komaroff, J Lieberman, "Silencing Bad Genes," In Newsweek Spacial Edition, The Future of Medicine: Your Health in the 21st Century, 51-52, 2005.

H Feng, D Zhang, D Palliser, P Zhu, S Cai, A Schlesinger, L Maliszewski, J Lieberman, "Listeria-infected myeloid dendritic cells produce IFN-beta, priming T cell activation," J Immunol 175:421-32, 2005. [PDF]

L Liu, Z Xu, RC Fuhlbrigge, V Pena-Cruz, J Lieberman, TS Kupper, "Vaccinia virus induces strong immunoregulatory cytokine production in healthy human epidermal keratinocytes: a novel strategy for immune evasion," J Virol 79:7363-70, 2005. [PDF]

E Song, P Zhu, SK Lee, D Chowdhury, S Kussman, DM Dykxhoorn, Y Feng, D Palliser, DB Weiner, P Shankar, WA Marasco, J Lieberman, "Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors," Nat Biotechnol 23:709-17, 2005. [PDF]

L Shi, D Keefe, E Durand, H Feng, D Zhang, J Lieberman, "Granzyme B binds to target cells mostly by charge and must be added at the same time as perforin to trigger apoptosis," J Immunol 174:5456-61, 2005. [PDF]

D Martinvalet, P Zhu, J Lieberman, "Granzyme A induces caspase-independent mitochondrial damage, a required first step for apoptosis," Immunity 22:355-70, 2005. [PDF]

P Shankar, N Manjunath, J Lieberman, "The prospect of silencing disease using RNA interference," JAMA 293:1367-73, 2005.

DM Dykxhoorn, J Lieberman, "The silent revolution: RNA interference as basic biology, research tool, and therapeutic," Annu Rev Med 6:401-23, 2005. [PDF]

P Shankar, J Lieberman, "RNAi and HIV: from here to therapy," in S. Butera, editor, HIV Chemotherapy: A Critical Review, Horizon Scientific Press, 2005.

Y Xue, JS Johnson, DA Ornelles, J Lieberman, DA Engel, "Adenovirus protein VII functions throughout early phase and interacts with cellular proteins SET and pp32," J Virol 79:2474-2483, 2005. [PDF]

V Francois-Bongarcon, S-K Lee, G Chen, P Shankar, Y Liu, X Tao, Y Shao, J Lieberman,  "Cross-clade CD8 T-cell responses to HIV IIIB and Chinese B' and C/B' viruses in North American and Chinese HIV seropositive donors," JAIDS 37:1435-1444, 2004. [Abstract]

MN Ince, B Harnisch, Z Xu, SK Lee, C Lange, L Moretta, M Lederman, J Lieberman, "Increased expression of the natural killer cell inhibitory receptor CD85j/ILT2 on antigen-specific effector CD8 T cells and its impact on CD8 T-cell function," Immunology 112:531-42, 2004. [PDF]

P Hamar, E Song, G Kokeny, A Chen, N Ouyang, J Lieberman, "Small interfering RNA targeting Fas protects mice against renal ischemia-reperfusion injury," Proc Natl Acad Sci USA 101:14883-8, 2004. [PDF]

J Lieberman, "Tracking the Killers: How should we measure CD8 T cells in HIV infection?" AIDS 18:1489-93, 2004. [PDF]

CG Lange, Z Xu, BK Patterson, K Medvik, B Harnisch, R Asaad, H Valdez, SJ Lee, A Landay, J Lieberman, MM Lederman, "Proliferation responses to HIV during antiretroviral therapy do not reflect improved immune phenotype or function," AIDS 18:605-13, 2004. [PDF]

DR Burton , RC Desrosiers, RW Doms, MB Feinberg, RC Gallo, B Hahn, JA Hoxie, E Hunter, B Korber, A Landay, MM Lederman, J Lieberman, JM McCune, JP Moore, N Nathanson, L Picker, D Richman, C Rinaldo, M Stevenson, DI Watkins , SM Wolinksky, JA Zack, "A sound rationale needed for phase III HIV-1 vaccine trials," Science 303:316, 2004. [PDF]

SK Lee, Z Xu, J Lieberman, P Shankar, "The functional CD8 T cell response to HIV becomes type-specific in progressive disease," J Clin Invest 110:1339-1347, 2002. [PDF]

J Lieberman, "Defying Death: HIV Mutatation to Evade Cytotoxic T Lymphocytes," New Engl J Med 347:1203-1204, 2002. [PDF]

CD Novina, MF Murray, DM Dykxhoorn, PJ Beresford, J Riess, S-K Lee, RG Collman, J Lieberman, P Shankar, PA Sharp, "siRNA-directed inhibition of HIV-1 infection," Nature Med 8:681-686, 2002. [PDF]

JLieberman, N Manjunath, P Shankar, "Avoiding the kiss of death: how HIV and other chronic viruses survive," Curr Opin Immunol 14:478-486, 2002. [PDF]

Z Fan, PJ Beresford, D Zhang, J Lieberman, "HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T lymphocyte protease granzyme A," Mol Cell Biol 22:2810-2820, 2002. [PDF]

J Lieberman, P Shankar, N Manjunath, J Andersson, "HIV infection of CD8 T cells: a factor in progressive immunodeficiency?" Blood 99:1877, 2002. [PDF]

J Lieberman, P Shankar, N Manjunath, J Andersson, "Dressed to kill? A review of why antiviral CD8 T lymphocytes fail to prevent progressive immunodeficiency in HIV-1 infection," Blood 98:1667-1677, 2001. [PDF]

PJ Beresford, D Zhang, DY Oh, Z Fan, EL Greer, ML Russo, M Jaju, J Lieberman, "Granzyme A Activates an Endoplasmic Reticulum-associated Caspase-independent Nuclease to Induce Single-stranded DNA Nicks," J Biol Chem 276:43285-43293, 2001. [PDF]

N Manjunath, P Shankar, J Wan, W Weninger, MA Crowley, K Hieshima, TA Springer, X Fan, H Shen, J Lieberman, UH von Andrian, "Effector differentiation is not prerequisite for generation of memory cytotoxic T lymphocytes," J Clin Invest 108:871-878, 2001. [PDF]

G Chen, P Shankar, C Lange, H Valdez, PR Skolnik, L Wu, N Manjunath, J Lieberman, "CD8 T cells specific for human immunodeficiency virus, Epstein-Barr virus, and cytomegalovirus lack molecules for homing to lymphoid sites of infection," Blood 98:156-164, 2001. [PDF]

E Sharif-Askari, A Alam, E Rheaume, PJ Beresford, C Scotto, K Sharma, D Lee, WE DeWolf, ME Nuttall, J Lieberman, R-P Sekaly, "Direct cleavage of the human DNA fragmentation factor-45 by granzyme B induces caspase-activated DNase release and DNA fragmentation," EMBO J 20:3101-3113, 2001. [PDF]

D Zhang, PJ Beresford, AH Greenberg, J Lieberman, "Granzymes A and B directly cleave lamins and disrupt the nuclear lamina during granule-mediated cytolysis," Proc Natl Acad Sci USA 98:5746-5751, 2001. [PDF]

D Zhang, MS Pasternack, PJ Beresford, L Wagner, AH Greenberg, J Lieberman, "Induction of rapid histone degradation by the cytotoxic T lymphocyte protease granzyme A," J Biol Chem 276:3683-3690, 2001. [PDF]

RS Friedman, FR Frankel, Z Xu, J Lieberman, "Induction of human immunodeficiency virus (HIV) -specific CD8 T-cell responses by Listeria monocytogenes and a hyperattenuated Listeria strain engineered to express HIV antigens," J Virol 74:9987-9993, 2000. [PDF]

P Shankar, M Russo, B Harnisch, M Patterson, P Skolnik, J Lieberman, "Impaired function of circulating HIV-specific CD8+ T cells in chronic human immunodeficiency virus infection," Blood 96:3094-3101, 2000. [PDF]

LA Trimble, P Shankar, M Patterson, JP Daily, J Lieberman, "Human immunodeficiency virus-specific circulating CD8 T lymphocytes have down-modulated CD3zeta and CD28, key signaling molecules for T cell activation," J Virol 74:7320-7330, 2000. [PDF]

LA Trimble, LW Kam, R FriedmanS, Z Xu, J Lieberman, "CD3zeta and CD28 down-modulation on CD8 T cells during viral infection," Blood 96:1021-1029, 2000. [PDF]

Y Lu, R Friedman, N Kushner, A Doling, L Thomas, N Touzjian, M Starnbach, J Lieberman, "Genetically modified anthrax lethal toxin safely delivers whole HIV protein antigens into the cytosol to induce T cell immunity," Proc Natl Acad Sci USA 97:8027-8032, 2000. [PDF]

CE Mackewicz, J Lieberman, C Froelich, JA Levy, "HIV virions and HIV infection in vitro are unaffected by human granzymes A and B," AIDS Res Hum Retroviruses 16:367-372, 2000. [Abstract]

J Lieberman, "Cytotoxic T lymphocyte adoptive immunotherapy for HIV infection," In: PA Henkart, MV Sitkovsky, editors, Cytotoxic Cells: Basic Mechanisms and Medical Applications. Philadelphia: Lippincott, Williams and Wilkins, 441-457, 2000.

F Lori, H Jessen, J Lieberman, D Finzi, E Rosenberg, C Tinelli, B Walker, RF Siliciano, J Lisziewicz, "Treatment of human immunodeficiency virus infection with hydroxyurea, didanosine, and a protease inhibitor before seroconversion is associated with normalized immune parameters and limited viral reservoir," J Infect Dis 180:1827-1832, 1999. [PDF]

N Manjunath, P Shankar, B Stockton, PD Dubey, J Lieberman, UH von Andrian, "A transgenic mouse model to analyze CD8+ effector T cell differentiation in vivo," Proc Natl Acad Sci USA 96:13932-13937, 1999. [PDF]

F Lori, E Rosenberg, J Lieberman, A Foli, R Maserati, E Seminari, F Alberici, B Walker, J Lisziewicz, "Hydroxyurea and didanosine long-term treatment prevents HIV breakthrough and normalizes immune parameters," AIDS Res Hum Retroviruses 15:1333-1338, 1999. [Abstract]

P Shankar, Z Xu, J Lieberman, "Viral-specific cytotoxic T lymphocytes lyse HIV-infected primary T lymphocytes by the granule exocytosis pathway," Blood 94:3084-3093, 1999. [PDF]

J Andersson, H Behbahani, J Lieberman, E Connick, A Landay, B Patterson, A Sonnerborg, K Lore, S Uccini, TE Fehniger, "Perforin is not co-expressed with granzyme A within cytotoxic granules in CD8 T lymphocytes present in lymphoid tissue during chronic HIV infection," AIDS 13:1295-1303, 1999. [Abstract]

F Lori, H Jessen, J Lieberman, M Clerici, C Tinelli, J Lisziewicz, "Immune restoration by combination of a cytostatic drug (hydroxyurea) and anti-HIV drugs (didanosine and indinavir)," AIDS Res Hum Retroviruses 15:619-624, 1999. [Abstract]

PJ Beresford, Z Xia, AH Greenberg, J Lieberman, "Granzyme A loading induces rapid cytolysis and a novel form of DNA damage independently of caspase activation," Immunity 10:585-594, 1999. [PDF]

J Lisziewicz, E Rosenberg, J Lieberman, H Jessen, L Lopalco, R Siliciano, B Walker, F Lori, "Control of HIV despite the discontinuation of antiretroviral therapy," New Engl J Med 340:1683-1684, 1999. [Abstract]

LA Trimble, Z Xu, J Lieberman, "Clonal expansion of antigen-specific CD8+ cytotoxic T lymphocytes is regulated by late exposure to serum to prevent apoptosis," J Immunol Meth 225:39-52, 1999. [PDF]

J Lieberman, LA Trimble, RS Friedman, J Lisziewicz, FLori, P Shankar, H Jessen, "Expansion of CD57 and CD62L-CD45RA+ CD8 T lymphocytes correlates with reduced viral plasma RNA after primary HIV infection," AIDS 13:891-899, 1999. [Abstract]

G MacDonald, L Shi, C Vande Velde, J Lieberman, AH Greenberg, "Mitochondria-dependent and -independent regulation of granzyme B-induced apoptosis," J Exp Med 189:131-143, 1999. [PDF]

P Shankar, H Sprang, J Lieberman, "Effective lysis of HIV-1 infected primary CD4+ T cells by a cytotoxic T lymphocyte clone directed against a novel A2-restricted reverse-transcriptase epitope," JAIDS 19:111-120, 1998. [Abstract]

PJ Beresford, M Jaju, RS Friedman, MJ Yoon, J Lieberman, "A role for heat shock protein 27 in CTL-mediated cell death," J Immunol 161:161-167, 1998. [PDF]

Z Xia, C-M Kam, C Huang, JC Powers, RJ Mandle, RL Stevens, J Lieberman, "Expression and purification of enzymatically active recombinant granzyme B in a baculovirus system," Biochem Biophys Res Comm 243:384-389, 1998. [PDF]

LA Trimble, J Lieberman, "Circulating CD8 T lymphocytes in human immunodeficiency virus-infected individuals have impaired function and downmodulate CD3zeta, the signaling chain of the T cell receptor complex," Blood 91:585-594, 1998. [PDF]

PJ Beresford, C-M Kam, JC Powers, J Lieberman, "Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them," Proc Natl Acad Sci USA 94:9285-9290, 1997. [PDF]

J Lieberman, PR Skolnik, GR Parkerson III, JA Fabry, B Landry, J Bethel, J Kagan, the DATRI 006 Study Team, "Safety of autologous, ex vivo-expanded HIV-specific cytotoxic T lymphocyte infusion in HIV-infected patients," Blood 90:2196-2206, 1997. [PDF]

J Lieberman, JA Fabry, DM Fong, GR Parkerson III, "Recognition of a small number of diverse epitopes dominates the cytotoxic T lymphocyte response to HIV type 1 in an infected individual," AIDS Res Hum Retroviruses 13:381-392, 1997. [Abstract]

RJ Buchsbaum, JA Fabry, J Lieberman, "EBV-specific cytotoxic T lymphocytes protect against human EBV-associated lymphoma in scid mice," Immunol Letters 52:145-152, 1996. [PDF]

P Shankar, JA Fabry, DM Fong, J Lieberman, "Three regions of HIV-1 gp160 contain clusters of immunodominant CTL epitopes," Immunol Letters 52:23-30, 1996. [PDF]

LL Decker, P Shankar, G Khan, RB Freeman, BJ Dezube, J Lieberman, DA Thorley-Lawson, "The Kaposi Sarcoma-associated Herpesvirus (KSHV) is present as an intact latent genome in KS tissue but replicates in the peripheral blood mononuclear cells of KS patients," J Exp Med 184:283-288, 1996. [PDF]

L Trimble, M-A Perales, R Knazek, J Lieberman, "Serum enhances the ex vivo generation of HIV-specific cytotoxic T cells," Biotech Bioengin 50:521-528, 1996. [Abstract]

M-A Perales, PR Skolnik, J Lieberman, "Effect of Interleukin 12 on in vitro HIV type 1 replication depends on clinical stage," AIDS Res Hum Retroviruses 12:659-668, 1996. [Abstract]

CGP Roberts, GE Meister, BM Jesdale, J Lieberman, JA Berzofsky, AS DeGroot, "Prediction of HIV peptide epitopes by a novel algorithm," AIDS Res Hum Retroviruses 12:593-610, 1996. [Abstract]

FR Frankel, S Hegde, J Lieberman, Y Paterson, "Induction of cell-mediated immune responses to human immunodeficiency virus type 1 Gag protein using Listeria monocytogenes as a live vaccine vector," J Immunol 155:4775-4782, 1995. [PDF]

M-A Perales, DH Schwartz, JA Fabry, J Lieberman, "A Vaccinia-gp160-based vaccine but not a gp160 protein vaccine elicits anti-gp160 cytotoxic T lymphocytes in some HIV-1 seronegative vaccinees," JAIDS 10:27-35, 1995. [Abstract]

RV Srinivas, T Su, LA Trimble, J Lieberman, B Ardman, "Enhanced susceptibility to human immunodeficiency virus infection in CD4+ T lymphocytes genetically deficient in CD43," AIDS Res Hum Retroviruses 11:1015-1021, 1995. [Abstract]

P Shankar, J Fabry, J Lieberman, "A simple method to selectively expand HIV-1 specific cytotoxic T lymphocytes in vitro," Immunol Investigations 24:489-497, 1995. [Abstract]

TA McFarland, B Ardman, N Manjunath, JA Fabry, J Lieberman, "CD43 diminishes susceptibility to T lymphocyte-mediated cytolysis," J Immunol 154:1097-1104, 1995. [PDF]

J Lieberman, JA Fabry, P Shankar, L Beckett, PR Skolnik, "Ex vivo expansion of HIV type-1 specific cytotoxic T cells from HIV type-1 seropositive subjects," AIDS Res Hum Retroviruses 11:257-271, 1995. [Abstract]